Bacterial Protein Expression (E. coli)

Availability: In stock

$84.00

Quick Overview

Single colony used to grow up 1 liter culture.
Bacterial pellet produced.
For further processing also select microfluidization of bacterial pellet and applicable protein purification.

Bacterial Protein Expression (E. coli)

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$84.00

Details

Single colony used to grow up 1 liter culture. Bacterial pellet produced. For further processing also select microfluidization of bacterial pellet and applicable protein purification. We recommend using the BL21 E. coli expression strains The basic BL21 strain does not contain the T7 RNA polymerase gene and can be used with non-T7 RNA polymerase protein expression systems. This strain provides the tightest control of protein expression for extremely toxic proteins. BL21-Gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene encoding endonuclease I (endA) inactivated. Together, these two features allow direct cloning for most protein expression constructs. By cloning directly in the strain, you can save 2 days of work normally spent on subcloning procedures. The DE3 pLysS-derivatives of BL21 contain the pLysS plasmid codes for T7 lysozyme, a natural inhibitor of T7 RNA polymerase. This derivative provides tighter control for expression of toxic proteins.